The reliable air tight seal and the fast gas exchange properties of the
CO2 Incubator,
allow for the rapid and economical creation of a non-fluctuating hypoxic environment.
Hypoxia environments are easily achieved using the CO2 Incubator.
Simple to Use:
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Place your tissue culture cells in the Chamber. |
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Attach flow meter to unit. |
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Flush for several minutes with desired gas mixture. |
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Seal the Chamber and place at 37oC in your laboratory incubator or warm room. |
Once sealed, this is a closed air tight system. The two gas ports on the Chamber can be easily modified to allow
monitoring of the pO2 inside the Chamber. Hypoxia in a publication (JBC 270:31189, 1995) a hypoxic environment was
created and maintained using the Chamber and the following protocol:
A pre-analyzed gas mixture of 5% CO2 and 95% N2 was flushed through the CO2 Incubator, at the flow rate
of 3 liters per min for 15 min twice a day. The pO2 reached a nadir of 35 mmHg after 6 h and remained constant the
remainder of the experiment (48 h). To test for hypoxia specific regulation of gene expression, cell cultures were
first exposed to pO2-35 mmHg for 24h and then returned to a normoxic environment of atmospheric air/5%CO2 at pO2=150 mmHg.
The pO2 was measured with a gas analyzer (278 Blood Gas Systems).
One-year warranty against leaks, defects and breakage.
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